Prostatic steroid-binding protein. Isolation and characterization of C3 genes.

Steroid-protein interactions. XV. Isolation and characterization of corticosteroid-binding globulin from human plasma.

Corticosteroid-binding globulin was isolated from normal human plasma by successive anion exchange chromatography and gel filtration. Ammonium sulfate fractionation was employed as a preliminary step when large volumes were processed. The purification was about 4000-fold. Homogeneity was shown by free boundary, agar and paper electrophoresis, sedimentation velocity, and diffusion. Immunoelectro...

Intronic steroid response elements in prostate binding protein genes.

Abbreviations used: GRE, glucocorticoid response element; ARE, androgen response element; MMTV, mouse mammary tumour virus; LTR, long terminal repeat. (MMTV) long terminal repeat (LTR) [2-41 or the tyrosine aminotransferase gene [ 51. However, androgen response elements (ARE) in androgen-responsive genes have been less easy to delimit. High-affinity androgen-receptor-binding regions have been d...

Isolation and Characterization of a Mannan-binding Protein

A binding protein from liver which binds reversibly to yeast mannan depending on the presence or absence of calcium has been purified to near homogeneity by affinity chromatography. The binding of the isolated protein to Y-mannan is proportional to protein concentration and is apparently an unsaturable process. The Scatchard plot of the binding is a curvilinear, indicating the presence of a hig...

Isolation and partial characterization of a glial hyaluronate-binding protein.

A glial hyaluronate-binding protein (GHAP) with an isoelectric point of 4.3-4.4 was isolated from human brain white matter. The 60-kDa glycoprotein appeared to be quite resistant to proteolysis, and comparison with GHAP from a viable glioma removed at surgery showed that the protein isolated from autopsy material was not a degradation product resulting from postmortem autolysis. The protein was...

Prostatitis in the Nonobese Diabetic Mouse Protein Peptide-Induced Autoimmune Spontaneous and Prostatic Steroid Binding